Rozee K R, Cooper D, Lam K, Costerton J W
Appl Environ Microbiol. 1982 Jun;43(6):1451-63. doi: 10.1128/aem.43.6.1451-1463.1982.
We have developed new methods to minimize fluid shear during preparation of specimens for electron microscopy and to retain the mucous blanket that covers the tissue surface of the ileum in mice. We also used general stabilization by nonspecific antibodies to minimize the collapse of the mucous layer during dehydration for electron microscopy. These methods allowed us to visualize the gradual progression of the mucous blanket from a thin diaphanous layer in newborn animals to a very thick (ca. 50 micrometers), coherent structure in older animals that contained a mixed population of bacteria and protozoa. Some bacteria, notably filamentous forms, were patently anchored to the epithelial tissue but projected into the mucous blanket, whereas others clearly existed within the mucous blanket and were unattached to the epithelial surface. Similarly, some protozoa were firmly attached to the tissue surface, whereas others were suspended in the viscous mucous blanket. In an adult animal, the mucous blanket was a very thick layer which actually occluded most of the tissue surface and contained a rich variety of bacteria and protozoa.
我们开发了新方法，以在制备用于电子显微镜检查的标本时尽量减少流体剪切力，并保留覆盖小鼠回肠组织表面的黏液层。我们还使用非特异性抗体进行一般稳定化处理，以尽量减少脱水过程中黏液层在电子显微镜检查时的塌陷。这些方法使我们能够观察到黏液层从新生动物中的薄而透明的层逐渐发展为老年动物中非常厚（约50微米）、连贯的结构，其中包含细菌和原生动物的混合群体。一些细菌，特别是丝状形式，明显锚定在上皮组织上，但伸入黏液层，而其他细菌则明显存在于黏液层内，未附着在上皮表面。同样，一些原生动物牢固地附着在组织表面，而其他原生动物则悬浮在粘性黏液层中。在成年动物中，黏液层是一层非常厚的层，实际上覆盖了大部分组织表面，并包含丰富多样的细菌和原生动物。
