Sandberg H, Andersson L O, Höglund S
Biochem J. 1982 Apr 1;203(1):303-11. doi: 10.1042/bj2030303.
Lipid-protein particles with platelet factor 3 measured by the Stypven clotting-time test [Hardisty & Hutton (1966) Br. J. Haematol. 12, 764-776] have been isolated from platelet-release supernatant. Starting material was washed platelets, which were released by treatment with collagen. Purification of the particles from other components in the release material was accomplished by gel filtration on Sepharose CL-4B followed by affinity chromatography on poly-L-lysine-Sepharose CL-4B gel. Chemical characterization showed that the particles were composed of 40% protein, 42% phospholipids, 13% cholesterol and 5% triacylglycerols. The phospholipid composition was 38% phosphatidylcholine, 25% phosphatidylethanolamine, 9% phosphatidylserine, 2% phosphatidic acid and 26% sphingomyelin. No carbohydrate was detected. Electron-microscopic studies revealed the presence of membranous particles with diameters between 70 and 170 nm.
已从血小板释放上清液中分离出通过Stypven凝血时间试验[哈迪斯蒂和赫顿（1966年），《英国血液学杂志》12卷，764 - 776页]测定的含血小板因子3的脂蛋白颗粒。起始材料是经胶原处理后释放的洗涤血小板。通过在Sepharose CL - 4B上进行凝胶过滤，随后在聚-L-赖氨酸-Sepharose CL - 4B凝胶上进行亲和色谱，从释放材料中的其他成分中纯化这些颗粒。化学表征表明，这些颗粒由40%的蛋白质、42%的磷脂、13%的胆固醇和5%的三酰甘油组成。磷脂组成是38%的磷脂酰胆碱、25%的磷脂酰乙醇胺、9%的磷脂酰丝氨酸、2%的磷脂酸和26%的鞘磷脂。未检测到碳水化合物。电子显微镜研究显示存在直径在70至170纳米之间的膜状颗粒。
