Dwyer J D, Bloomfield V A
Biochemistry. 1982 Jun 22;21(13):3227-31. doi: 10.1021/bi00256a030.
Quasi-elastic laser light scattering (QLS) is used to study the translational frictional properties of cholera toxin and its complex with ganglioside Gm1 receptor containing phospholipid vesicles. These properties are compared to theoretically calculated values for model structures composed of spherical subunits in order to assess the structural configuration of the toxin and its binding geometry on membrane surfaces. The structure for the toxin that best fits the experimental results consists of the five B subunits arranged radially about an elongated A subunit, which extends well above the plane of the B subunits. Binding of cholera toxin to Gm1-containing model membranes results in a complex in which the B subunits are absorbed on the surface while the A subunit penetrates the membrane bilayer.
准弹性激光光散射（QLS）用于研究霍乱毒素及其与含神经节苷脂Gm1受体的磷脂囊泡复合物的平移摩擦特性。将这些特性与由球形亚基组成的模型结构的理论计算值进行比较，以评估毒素的结构构型及其在膜表面的结合几何形状。最符合实验结果的毒素结构是由五个B亚基围绕一个细长的A亚基呈放射状排列，A亚基延伸到B亚基平面上方很远的位置。霍乱毒素与含Gm1的模型膜结合形成一种复合物，其中B亚基吸附在表面，而A亚基穿透膜双层。
