Katchman A N, Zeimal E V
Brain Res. 1982 Jun 3;241(1):95-103. doi: 10.1016/0006-8993(82)91232-x.
Current responses to acetylcholine (ACh) and to suberyldicholine (D-6) applied from the double-barrelled ionophoretic micropipette were studied in two identified neurones (LPed-2 and LPed-3) isolated from the left ganglion of pulmonate mollusc, Planorbarius corneus. Experiments made with K2SO4-filled microelectrodes show that in LPed-2 neurone two kinds of cholinoreceptors are involved in the rapid phase of ACh response one of which induces chloride conductance and the other, sodium conductance. The Cl-dependent component can be separated from the cationic one by C-6 whereas the cationic component can be separated from the Cl--dependent one by furosemide. Cl- conductance can be induced selectively by D-6. In the LPed-3 neurone only Cl- conductance increases during rapid phase of ACh response. The reversal potential of Cl--dependent responses was found to be more negative than the resting potential in experiments made with K2SO4-filled microelectrodes but less negative than the resting potential in the case of KCl-filled microelectrodes. This difference seems to be due to the artificial increase of intracellular chloride concentration.
在从肺螺软体动物角贝（Planorbarius corneus）左神经节分离出的两个已鉴定神经元（LPed - 2和LPed - 3）中，研究了从双管离子电泳微吸管施加的乙酰胆碱（ACh）和辛二酰二胆碱（D - 6）的当前反应。用填充硫酸钾的微电极进行的实验表明，在LPed - 2神经元中，两种胆碱能受体参与了ACh反应的快速阶段，其中一种诱导氯离子电导，另一种诱导钠离子电导。依赖氯离子的成分可以通过C - 6与阳离子成分分离，而阳离子成分可以通过速尿与依赖氯离子的成分分离。D - 6可以选择性地诱导氯离子电导。在LPed - 3神经元中，在ACh反应的快速阶段仅氯离子电导增加。在用填充硫酸钾的微电极进行的实验中，发现依赖氯离子反应的反转电位比静息电位更负，但在用填充氯化钾的微电极的情况下，比静息电位负性更小。这种差异似乎是由于细胞内氯离子浓度的人为增加。
