Saper C B
Brain Res. 1982 Jun 17;242(1):33-40. doi: 10.1016/0006-8993(82)90493-0.
The projection from the parabrachial nucleus (PB) to the cerebral cortex in the rat was studied in detail using the autoradiographic method for tracing anterograde axonal transport and the wheat germ agglutinin-horseradish peroxidase (WGA-HRP) method for both anterograde and retrograde tracing. PB innervates layers I, V and VI of a continuous sheet of cortex extending from the posterior insular cortex caudally, through the dorsal agranular and the granular anterior insular cortex and on rostrally into the lateral prefrontal cortex. Within the prefrontal area, PB fibers innervate primarily layer V of the ventrolateral cortex caudally, but more rostrally the innervated region includes progressively more dorsal portions of the prefrontal area, until by the frontal pole the entire lateral half of the hemisphere is innervated. This projection originates for the most part in a cluster of neurons in the caudal ventral part of the medial PB subdivision, although a few neurons in the adjacent parts of the PB, the Kolliker-Fuse nucleus and the subcoeruleus region also participate. After injection of WGA-HRP into the PB region, retrogradely labeled neurons were found in layer V of the same cortical areas which receive PB inputs. The importance of this monosynaptic reciprocal brainstem-cortical projection as a possible anatomical substrate for the regulation of cortical arousal is discussed.
采用放射性自显影法追踪顺行轴突运输，以及利用小麦胚凝集素 - 辣根过氧化物酶（WGA - HRP）法进行顺行和逆行追踪，对大鼠臂旁核（PB）向大脑皮层的投射进行了详细研究。PB支配着从后岛叶皮层向后延伸的连续皮层片的I、V和VI层，穿过背侧无颗粒和颗粒状前岛叶皮层，并向前延伸至外侧前额叶皮层。在前额叶区域内，PB纤维主要支配尾侧腹外侧皮层的V层，但在更靠前的区域，支配区域逐渐包括前额叶区域更多的背侧部分，直到额叶极，半球的整个外侧半部分都有神经支配。这种投射大部分起源于内侧PB亚区尾侧腹侧部分的一群神经元，尽管PB相邻部分、柯利克 - 富斯核和蓝斑下区域的一些神经元也参与其中。将WGA - HRP注入PB区域后，在接受PB输入的相同皮层区域的V层中发现了逆行标记的神经元。讨论了这种单突触相互的脑干 - 皮层投射作为调节皮层觉醒的可能解剖学基础的重要性。
