Caviness V S
Brain Res. 1982 Jul;256(3):293-302. doi: 10.1016/0165-3806(82)90141-9.
The relative positions of the principal neuronal classes of neocortex are inverted in the reeler mutant mouse. Neurons formed at 48-hourly intervals throughout the period of neocortical cytogenesis between E11 and E17 are labeled by [3H]thymidine. The positions of the labeled cells during and subsequent to their migrations are traced by autoradiography. Simultaneously-formed cohorts reach the neocortex at the same time in normal and reeler animals. After E13, subsequent to the appearance of the cortical plate, cohorts of migrating cells in the normal animal ascend to the interface of the cortical plate and marginal layer where they come to rest in a narrow laminar zone. In reeler, by contrast, migration is arrested in the depths of the cortex. The migrating cell appears unable to ascend through the zone occupied by the preceding cohorts. At the completion of migration neurons of both genotypes become fixed in position and undergo little subsequent shift in their relative positions in the course of future cortical growth. Despite the anomaly of migrations and the post-migratory positions of neurons in reeler, cohorts of cells formed at the same time in the two genotypes give rise to the same neuronal classes.
在reeler突变小鼠中，新皮层主要神经元类别的相对位置发生了倒置。在胚胎第11天（E11）至第17天（E17）整个新皮层细胞发生期，每隔48小时形成的神经元用[3H]胸腺嘧啶核苷进行标记。通过放射自显影追踪标记细胞在迁移过程中及迁移后的位置。在正常动物和reeler动物中，同时形成的细胞群同时到达新皮层。在E13之后，即在皮质板出现之后，正常动物中迁移的细胞群上升到皮质板和边缘层的界面，在那里它们停留在一个狭窄的层状区域。相比之下，在reeler小鼠中，迁移在皮质深处受阻。迁移的细胞似乎无法穿过先前细胞群占据的区域向上迁移。在迁移完成时，两种基因型的神经元都固定在各自的位置，并且在未来皮质生长过程中其相对位置几乎没有后续变化。尽管reeler小鼠中存在迁移异常和神经元迁移后的位置异常，但两种基因型中同时形成的细胞群产生相同的神经元类别。
