Baszczynski C L, Walden D B, Atkinson B G
Can J Biochem. 1982 May;60(5):569-79. doi: 10.1139/o82-070.
Subjecting 5-day-old plumules of corn (Zea mays L.) to elevated temperatures for brief periods of time causes the pattern of protein synthesis to shift from the production of a broad spectrum of proteins to the new and (or) enhanced synthesis of a small number of heat-shock polypeptides (HSPs). Most notable is the depressed synthesis of a major polypeptide (relative mass (Mr) = 93 000 and isoelectric point = 8.0) normally made at 27 degrees C and the enhanced and (or) new synthesis of polypeptides with MrS of 108 000, 89 000, 84 000, 76 000, 73 000, and 18 000, following 1 h of heat shock. These six HSPs is observed within 120 min following heat shock. Recovery from heat shock is rapid; after 6 to 8 h at 27 degrees C following heat shock, the polypeptide pattern is indistinguishable from the control. Extracts from individual heat-shocked shoots produced polypeptide synthetic patterns identical to those from extracts from 20 shoots, regardless of whether single shoots were intact or excised during labelling. Single 5-day-old primary roots exhibited polypeptide synthetic patterns and responded to heat shock in a manner similar to shoots. This is the first demonstration of the induction of heat-shock polypeptides in a whole, intact higher plant.
将5日龄的玉米（Zea mays L.）胚芽短时间置于高温环境中，会导致蛋白质合成模式从产生多种蛋白质转变为新合成和（或）增强合成少量热休克多肽（HSPs）。最显著的是，通常在27℃下合成的一种主要多肽（相对分子质量（Mr）= 93 000，等电点 = 8.0）的合成受到抑制，而在热休克1小时后，相对分子质量为108 000、89 000、84 000、76 000、73 000和18 000的多肽的合成增强和（或）重新开始。在热休克后120分钟内可观察到这六种热休克蛋白。热休克后的恢复很快；热休克后在27℃下放置6至8小时后，多肽模式与对照无法区分。无论在标记过程中单个芽是完整的还是切除的，来自单个热休克芽的提取物产生的多肽合成模式与来自20个芽的提取物相同。单个5日龄的初生根表现出与芽相似的多肽合成模式并对热休克作出反应。这是首次在完整的高等植物中证明热休克多肽的诱导。
