Atkinson B G, Pollock M
Can J Biochem. 1982 Mar;60(3):316-27. doi: 10.1139/o82-038.
The brief incubation of human epidermoid carcinoma (KB) cells, and of primary cultures of quail myoblasts and hamster fibroblasts, at an elevated temperature causes the pattern of gene expression to shift from the production of a broad spectrum of different proteins to the enhanced synthesis of a small number of heat-shock proteins. Comparison of the heat-shock polypeptides synthesized by each of these vertebrate cells demonstrates the similarity of some, as well as the uniqueness of other, heat-inducible gene products synthesized by cells from different vertebrates. A major polypeptide, commonly synthesized in response to heat by each of these vertebrate cells, has an apparent molecular weight of 64 000 and an isoelectric point of 5.8. Triton X-100 completely extracts this polypeptide from quail myoblasts and hamster fibroblasts, and partially extracts it from KB cells. This particular response to heat shock, by cells from different vertebrates, suggests that it may involve the expression of a gene(s) with an analogous and potentially crucial cellular function. This specific heat-shock polypeptide, as well as others, is not detectably synthesized in quail cells prior to heat shock or 6--8 h after recovery from heat shock which suggests that in this cell type it may be a product of a normally quiescent gene(s) and that its expression is subject to thermal regulation.
将人表皮样癌（KB）细胞、鹌鹑成肌细胞原代培养物和仓鼠成纤维细胞在高温下短暂孵育，会导致基因表达模式从产生多种不同蛋白质转变为少数热休克蛋白的合成增强。对这些脊椎动物细胞各自合成的热休克多肽进行比较，表明不同脊椎动物细胞合成的一些热诱导基因产物具有相似性，而另一些则具有独特性。这些脊椎动物细胞在受热时共同合成的一种主要多肽，其表观分子量为64000，等电点为5.8。Triton X-100能从鹌鹑成肌细胞和仓鼠成纤维细胞中完全提取这种多肽，从KB细胞中部分提取。不同脊椎动物细胞对热休克的这种特殊反应表明，它可能涉及具有类似且可能至关重要的细胞功能的一个或多个基因的表达。这种特定的热休克多肽以及其他多肽，在热休克前或从热休克恢复后6 - 8小时的鹌鹑细胞中未检测到合成，这表明在这种细胞类型中它可能是一个正常静止基因的产物，其表达受温度调节。
