Ito K, Morita T, Takeuchi T
Biological Institute, Tohoku University, Sendai, Japan.
Cell Struct Funct. 1988 Jun;13(3):267-70. doi: 10.1247/csf.13.267.
The purpose of the present study is to analyze the effect of serum or chick embryo extract (CEE) on the neuronal differentiation of the mouse neural crest cells. When the crest cells were cultured in the medium containing serum at low concentration (5% calf serum), neurite outgrowth was observed. The active outgrowth was detected at 3-4 days in culture. However, in the medium supplemented with 20% calf serum, no neurite appeared, and the crest cells remained fibroblast-like. The differentiation of adrenergic neurons was observed when the crest cells were cultured in the medium containing CEE along with serum.
本研究的目的是分析血清或鸡胚提取物（CEE）对小鼠神经嵴细胞神经元分化的影响。当嵴细胞在含有低浓度血清（5%小牛血清）的培养基中培养时，可观察到神经突生长。在培养3 - 4天时检测到活跃的生长。然而，在补充有20%小牛血清的培养基中，未出现神经突，嵴细胞仍保持成纤维细胞样。当嵴细胞在含有CEE和血清的培养基中培养时，可观察到肾上腺素能神经元的分化。
