Wheatley D N, Inglis M S
Cytobios. 1982;33(129):15-28.
The acid-extractable pool in mammalian cells is composed of amino acids bound to or complexed with other molecules, possible proteins. It does not appear to consist of free amino acid molecules restrained within the cell by a supposedly impermeable plasma membrane. Attempts to characterize the pool have involved permeabilizing cells to macromolecules by standard procedures, and extraction of amino acids by a variety of chemical and physical methods. The molecules seem to be held by covalent bonds of a partially ionic nature, or in the form of a complex which may involve more than one other moiety. Simple ionic and hydrogen bonding have been ruled out, and disulphide bonds are not involved. Pools can be as effectively extracted with the lower alcohols as with organic acids. Further characterization will require the exceedingly difficult task of releasing amino acids from cells without disrupting their links with other moieties. The findings are consistent with the behaviour of pools in relations to protein synthesis (Wheatley and Inglis, 1980), and may also explain differential discharge kinetics.
哺乳动物细胞中的酸可提取库由与其他分子（可能是蛋白质）结合或络合的氨基酸组成。它似乎不是由被假定不可渗透的质膜限制在细胞内的游离氨基酸分子组成。表征该库的尝试包括通过标准程序使细胞对大分子通透，并通过各种化学和物理方法提取氨基酸。这些分子似乎通过部分离子性质的共价键结合，或以可能涉及不止一个其他部分的复合物形式存在。简单的离子键和氢键已被排除，且不涉及二硫键。用低级醇与用有机酸一样能有效地提取库。进一步的表征将需要完成一项极其困难的任务，即从细胞中释放氨基酸而不破坏它们与其他部分的联系。这些发现与库在蛋白质合成方面的行为一致（惠特利和英格利斯，1980年），也可能解释差异释放动力学。
