Wheatley D N
Cytobios. 1981;31(122):117-34.
Experimental evidence is presented which eliminates the acid-extractable (acid-soluble) pool of amino acids within cells from being the source of precursor molecules for protein synthesis. This is equally valid for those amino acids initially showing quasi-exponential incorporation into protein, viz, glycine and proline, contrary to the reports of others (e.g. Robinson, 1977). Reexamination of the design of some of the experiments showing the pool acting as the precursor supply has revealed technical defects which may have been responsible for this erroneous conclusion. The acid-extractable pool can be more clearly understood as the accumulation of complexed amino acids which have passed the selection mechanism of the intracellular cyclical perfusion system (Wheatley and Inglis, 1980), but are no longer directly available for protein synthesis.
实验证据表明，细胞内酸可提取（酸溶性）氨基酸池并非蛋白质合成前体分子的来源。这对于最初显示出近似指数方式掺入蛋白质的氨基酸（即甘氨酸和脯氨酸）同样适用，这与其他人的报道（例如罗宾逊，1977年）相反。对一些表明该池作为前体供应的实验设计进行重新审视后发现了技术缺陷，这些缺陷可能是导致这一错误结论的原因。酸可提取池可以更清楚地理解为已通过细胞内循环灌注系统选择机制（惠特利和英格利斯，1980年）的复合氨基酸的积累，但不再直接用于蛋白质合成。
