Bier D M, Matthews D E
Fed Proc. 1982 Aug;41(10):2679-85.
During the last two decades, in parallel with the growth of modern electronics, several new techniques have been developed for measuring stable isotopic enrichments in biochemistry and medicine. The development and potential of these techniques are discussed. Of these methods, mass spectrometry has been developed and refined the fullest to quantitate stable isotope tracers in very minute samples and for very large dilutions of tracer. No single mass spectrometric technique can measure this entire range, and different techniques are used for different applications. Examples of the different methods are presented for determining whole-body amino acid and protein dynamics in humans with stable isotopically labeled amino acid tracers.
在过去二十年中，随着现代电子技术的发展，已经开发出几种用于测量生物化学和医学中稳定同位素丰度的新技术。本文讨论了这些技术的发展及其潜力。在这些方法中，质谱技术得到了最充分的发展和完善，可用于在非常微量的样品中以及对示踪剂进行非常大倍数稀释的情况下定量稳定同位素示踪剂。没有一种单一的质谱技术能够测量整个范围，不同的技术用于不同的应用。文中给出了使用稳定同位素标记的氨基酸示踪剂测定人体全身氨基酸和蛋白质动力学的不同方法的实例。
