Westin G, Visser L, Zabielski J, van Mansfeld A D, Pettersson U, Rozijn T H
Gene. 1982 Mar;17(3):263-70. doi: 10.1016/0378-1119(82)90142-1.
The hamster cell line BHK268-C31 contains two large viral inserts which both include sequences from the left-hand end of adenovirus type 5 (Ad5) DNA. One of these viral inserts has been cloned in the lambda vector Charon 4A. Electron microscopic analysis and restriction enzyme mapping shows that the recombinant carries a 4.4-kb-long colinear segment of viral DNA, which is located between map positions 1.5 and 14.2 in the Ad5 genome. The junctions between viral DNA and flanking sequences have been sequenced and found not to show any specific features. One of the junctions is located in the E1a coding region, 573 bp from the left-hand end of the Ad5 genome, whereas the other junction is situated in the coding region for polypeptide IVa2. The promoter region as well as the cap site for the mRNAs from region E1a are thus missing from this insert and its role in viral transformation is unclear.
仓鼠细胞系BHK268 - C31含有两个大型病毒插入片段，二者均包含来自5型腺病毒（Ad5）DNA左端的序列。其中一个病毒插入片段已克隆至λ载体Charon 4A中。电子显微镜分析和限制性内切酶图谱分析表明，该重组体携带一段4.4 kb长的病毒DNA共线性片段，其位于Ad5基因组图谱位置1.5和14.2之间。病毒DNA与侧翼序列之间的连接点已测序，未发现任何特异性特征。其中一个连接点位于E1a编码区，距Ad5基因组左端573 bp，而另一个连接点位于多肽IVa2的编码区。因此，该插入片段缺失了E1a区域mRNA的启动子区域以及帽位点，其在病毒转化中的作用尚不清楚。
