Kawarabayasi Y, Sugisaki H
Nucleic Acids Res. 1985 Sep 25;13(18):6591-604. doi: 10.1093/nar/13.18.6591.
A DNA segment carrying viral DNA was cloned from a rat cell line transformed by the cloned EcoRI-C fragment (0 to 16.4 map units) of human adenovirus type 12(Ad12), and the viral sequence in the clone was analysed. The cloned segment contained the region from nucleotide positions 118 to 3520 of the Ad12 genome in the middle. No unique structure was found at the viral and non-viral DNA junctions. When examined the transforming activity, the conserved viral sequence was able to transform rat 3Y1 cells efficiently. Southern blotting analysis of the viral sequence in five re-transformed cell lines showed that the viral sequence was inserted at different sites of cellular DNA. These results indicate that (I) the Ad12 DNA moiety from the enhancer-promoter region of the E1A gene to the end of the E1B gene contains enough information for efficient transformation of the rat cell, and (II) integration of the viral sequence at unique cellular sites is not prerequisite for transformation.
从经人12型腺病毒（Ad12）克隆的EcoRI-C片段（0至16.4个图谱单位）转化的大鼠细胞系中克隆出携带病毒DNA的DNA片段，并对克隆中的病毒序列进行了分析。克隆片段包含Ad12基因组中间从核苷酸位置118至3520的区域。在病毒和非病毒DNA连接处未发现独特结构。在检测转化活性时，保守的病毒序列能够高效转化大鼠3Y1细胞。对五个再次转化的细胞系中的病毒序列进行Southern印迹分析表明，病毒序列插入到细胞DNA的不同位点。这些结果表明：（I）从E1A基因的增强子-启动子区域到E1B基因末端的Ad12 DNA部分包含足够的信息以高效转化大鼠细胞；（II）病毒序列整合到独特的细胞位点不是转化的先决条件。
