Pavlásek I
Folia Parasitol (Praha). 1982;29(2):115-8.
Oocysts of coccidia of the genes Cryptosporidium were detected for the first time in the faeces of naturally infected calves using centrifugation floatation method with saturated solution of ZnSo4 and Breza's floatation solution. Since the detection of oocysts in the faeces is very difficult, a new method has been prepared. The oocysts recovered from the faeces were sporulated, spherical or slightly elliptical, with a smooth, colourless and thin wall. In the fresh preparation after floatation the oocysts measured 5.4 X 4.6 micrometer on the average, whereas in the smears stained after Giemsa, their average size was 4.8 X 4.3 micrometer. A typical feature of the oocysts is a residual body appearing as a dark point at one pole of the oocyst when observed by optical microscope. The oocyst contains four sporozoites forming a C-shaped rim around the residual body. No sporocysts were recorded. The residual body stains blue and the nucleus dark red by Giemsa's method.
使用硫酸锌饱和溶液和布雷扎浮选液的离心浮选法，首次在自然感染牛犊的粪便中检测到隐孢子虫属球虫的卵囊。由于在粪便中检测卵囊非常困难，因此制备了一种新方法。从粪便中回收的卵囊呈孢子化状态，球形或略呈椭圆形，壁光滑、无色且薄。浮选后的新鲜制剂中，卵囊平均尺寸为5.4×4.6微米，而吉姆萨染色涂片后，其平均尺寸为4.8×4.3微米。卵囊的一个典型特征是，在光学显微镜下观察时，残留体在卵囊的一极呈现为一个暗点。卵囊含有四个子孢子，围绕残留体形成一个C形边缘。未记录到孢子囊。用吉姆萨法染色，残留体呈蓝色，细胞核呈深红色。
