丙酮酸脱羧酶活性及丙酮酸浓度对卡尔斯伯酵母中1-羟基-1-苯基丙酮生成的影响。

Effect of pyruvate decarboxylase activity and of pyruvate concentration on the production of 1-hydroxy-1-phenylpropanone in Saccharomyces carlsbergensis.

作者信息

Vojtísek V, Netrval J

出版信息

Folia Microbiol (Praha). 1982;27(3):173-7. doi: 10.1007/BF02877396.

Abstract

The assumption that the pyruvate decarboxylase activity of Saccharomyces carlsbergensis is the main limiting factor determining the formation rate and the total amount of d(--)-l-hydroxy-l-phenyl-propanone (phenylacetylcarbinol, PAC) produced was not confirmed. An increase of about 30% of the total amount of the PAC produced was obtained when 8.5% sodium pyruvate was gradually added. The total PAC production is probably influenced both by the pyruvate decarboxylase activity and the pyruvate concentration in the cells, the latter being actually the determining rate-limiting factor.

摘要

认为卡尔斯伯酵母的丙酮酸脱羧酶活性是决定d(-)-l-羟基-l-苯基丙酮（苯乙酰甲醇，PAC）生成速率和总量的主要限制因素这一假设未得到证实。当逐渐添加8.5%的丙酮酸钠时，所产生的PAC总量增加了约30%。PAC的总产量可能受到丙酮酸脱羧酶活性和细胞中丙酮酸浓度的影响，而后者实际上是决定速率的限制因素。