Schmitt H D, Zimmermann F K
J Bacteriol. 1982 Sep;151(3):1146-52. doi: 10.1128/jb.151.3.1146-1152.1982.
Six different pyruvate decarboxylase mutants of Saccharomyces cerevisiae were isolated. They belong to two unlinked complementation groups. Evidence is presented that one group is affected in a structural gene. The fact that five of the six mutants had residual pyruvate decarboxylase activity provided the opportunity for an intensive physiological characterization. It was shown that the loss of enzyme activity in vitro is reflected in a lower fermentation rate, an increased pyruvate secretion, and slower growth on a 2% glucose medium. The different effects of antimycin A on leaky mutants grown on ethanol versus the same mutants grown on glucose support the view that glucose induces some of the glycolytic enzymes, especially pyruvate decarboxylase.
分离出了酿酒酵母的六种不同的丙酮酸脱羧酶突变体。它们属于两个不连锁的互补群。有证据表明其中一组在一个结构基因中受到影响。六个突变体中有五个具有残留的丙酮酸脱羧酶活性，这一事实为深入的生理学特征分析提供了机会。结果表明，体外酶活性的丧失反映在较低的发酵速率、丙酮酸分泌增加以及在2%葡萄糖培养基上生长较慢。抗霉素A对在乙醇上生长的渗漏突变体与在葡萄糖上生长的相同突变体的不同影响支持了葡萄糖诱导一些糖酵解酶，尤其是丙酮酸脱羧酶的观点。
