Reusch V M
J Bacteriol. 1982 Sep;151(3):1543-52. doi: 10.1128/jb.151.3.1543-1552.1982.
Sacculi were prepared from Streptococcus sanguis 34 by exhaustive extraction of bacteria with hot 1% sodium dodecyl sulfate-0.5% 2-mercaptoethanol. Lyophilized residue was dissociated by brief sonication to single bodies closely resembling streptococci in phase-contrast microscopic density, staining properties, and morphology. Electron micrographs revealed bodies that contained variable amounts of cellular contents and were bounded by intact cell walls. Chemical analyses of sacculi demonstrated the presence of peptidoglycan, carbohydrate, protein, and phosphate. The hexose content of sacculi varied 10-fold depending upon the composition of the growth medium. When sacculi were subjected to treatment with 5 M LiCl, 8 M urea, 40% phenol (25 degrees C), or dimethyl sulfoxide most of the nitrogen and carbohydrate present was recovered in the insoluble fraction. These data suggest that sacculi contain the cell wall fraction of the extracted bacteria and that most of the carbohydrates and proteins of sacculi are firmly bound to the insoluble fraction, which contains the peptidoglycan matrix.
通过用热的1%十二烷基硫酸钠-0.5% 2-巯基乙醇彻底提取血链球菌34来制备球囊。冻干残渣经短暂超声处理解离成单个物体，在相差显微镜下的密度、染色特性和形态与链球菌极为相似。电子显微镜照片显示，这些物体含有不同数量的细胞内容物，并被完整的细胞壁所包围。球囊的化学分析表明存在肽聚糖、碳水化合物、蛋白质和磷酸盐。球囊的己糖含量根据生长培养基的组成而变化10倍。当球囊用5M LiCl、8M尿素、40%苯酚（25℃）或二甲基亚砜处理时，大部分存在的氮和碳水化合物在不溶性部分中被回收。这些数据表明球囊包含提取细菌的细胞壁部分，并且球囊的大部分碳水化合物和蛋白质与包含肽聚糖基质的不溶性部分紧密结合。
