Liljemark W F, Bloomquist C G
Infect Immun. 1981 Nov;34(2):428-34. doi: 10.1128/iai.34.2.428-434.1981.
The isolation and partial characterization of a protein-containing cell surface component from Streptococcus sanguis which blocks the adherence of this microbe to saliva-coated hydroxyapatite are described. Several methods of extraction were attempted. Sonication of whole cells and cell walls proved to be the most successful and yielded biologically active adherence-blocking components. The adherence-blocking ability of these components was effective in intraspecies blocking experiments. The extract obtained from cell walls of S. sanguis was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and shown to contain one major and two to three minor bands when stained with Coomassie blue. The molecular weight of the major band was estimated to be 70,000 to 90,000. Gel filtration of the sonified cell wall extract on 10% agarose yielded two active adherence-blocking peaks, the void volume and a second peak.
本文描述了从血链球菌中分离出一种含蛋白质的细胞表面成分，并对其进行了部分特性鉴定，该成分可阻止这种微生物黏附于唾液包被的羟基磷灰石。尝试了几种提取方法。结果证明，对全细胞和细胞壁进行超声处理最为成功，可产生具有生物活性的黏附阻断成分。这些成分的黏附阻断能力在种内阻断实验中有效。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对从血链球菌细胞壁获得的提取物进行检测，用考马斯亮蓝染色后显示含有一条主要条带和两到三条次要条带。主要条带的分子量估计为70,000至90,000。将超声处理后的细胞壁提取物在10%琼脂糖上进行凝胶过滤，得到两个活性黏附阻断峰，即空体积峰和第二个峰。
