溴化乙锭与4',6-二脒基-2-苯基吲哚作为琼脂糖凝胶中DNA定量荧光染色剂的比较。

Comparison of ethidium bromide and 4'6-diamidino-2-phenylindole as quantitative fluorescent stains for DNA in agarose gels.

作者信息

Nairn R S, Dodson M L, Humphrey R M

出版信息

J Biochem Biophys Methods. 1982 Jun;6(2):95-103. doi: 10.1016/0165-022x(82)90055-0.

Abstract

Two methods of quantitative, fluorescent detection of DNA bands in agarose gels separated by electrophoresis were evaluated for sensitivity and linearity of response. Comparisons of ethidium bromide staining with a method using 4',6-diamidino-2-phenylindole (DAPI) developed in this work showed that DAPI is several times more sensitive than ethidium bromide in conditions of comparable background fluorescence. Optimum fluorescent staining and detection conditions for DNA bands in agarose gels using DAPI are described, and advantages of this method over other fluorescent detection methods are discussed.

摘要

对通过电泳分离的琼脂糖凝胶中DNA条带的两种定量荧光检测方法的灵敏度和响应线性进行了评估。将溴化乙锭染色与本研究中开发的使用4',6-二脒基-2-苯基吲哚（DAPI）的方法进行比较，结果表明，在背景荧光相当的条件下，DAPI的灵敏度比溴化乙锭高几倍。描述了使用DAPI对琼脂糖凝胶中DNA条带进行最佳荧光染色和检测的条件，并讨论了该方法相对于其他荧光检测方法的优势。