通过抑制游离e抗原的电泳来测定乙型肝炎e抗原抗体。

Determination of the antibody to hepatitis B e antigen by the inhibition on electrophoresis of free e antigen.

作者信息

Gotanda T, Imai M, Sano T, Oinuma A, Nomura M, Miyakawa Y, Mayumi M

出版信息

J Immunol Methods. 1982;51(3):347-53. doi: 10.1016/0022-1759(82)90402-1.

Abstract

A new method is described for the rapid and sensitive detection of the antibody to hepatitis B e antigen (anti-HBe) in serum. Twenty-five microliters of the test serum were incubated with 10 microliter of the purified small 'free' e antigen, and then the consumption of the added antigen was detected by the failure to precipitate with standard anti-HBe in counter-electrophoresis. When a total of 444 serums from asymptomatic carriers of hepatitis B surface antigen was tested for anti-HBe by this method, 348 (78.4%) were found to be positive with a detectability much higher than that of the passive hemagglutination method which detected the antibody in only 264 (59.5%) of the same serums.

摘要

本文描述了一种快速、灵敏地检测血清中乙型肝炎e抗原抗体（抗-HBe）的新方法。将25微升检测血清与10微升纯化的小“游离”e抗原一起孵育，然后通过在对流电泳中不能与标准抗-HBe沉淀来检测添加抗原的消耗情况。用该方法对总共444份乙型肝炎表面抗原无症状携带者的血清进行抗-HBe检测时，发现348份（78.4%）呈阳性，其检测能力远高于被动血凝法，被动血凝法在相同血清中仅检测出264份（59.5%）抗体阳性。