[来自犬小肠上皮细胞的微绒毛膜蛋白]

[Microvilli membrane proteins from dog enterocytes].

作者信息

Starita-Geribaldi M, Fehlmann M, Sudaka P

出版信息

Arch Int Physiol Biochim. 1977 Apr;85(2):245-54. doi: 10.3109/13813457709058757.

Abstract

Mcrovilli membranes have been isolated from dog jejunal and ileal enterocytes. Proteins were analysed by polyacrylamide gel electrophoresis after solubilization with sodium dodecylsulphate. The recovery of the membrane fraction with this purification method was found to be 22% and the specific activity of sucrase increases 19 folds in the membrane fraction. Microvilli membrane proteins after polyacrylamide gel electrophoresis were seprated in 21 bands, most of them with a molecular weight higher than 70 000. Seven bands with molecular weight from 150 000 to more than 340 000, were found to be glycoproteins.

摘要

微绒毛膜已从犬空肠和回肠肠细胞中分离出来。用十二烷基硫酸钠溶解后，通过聚丙烯酰胺凝胶电泳分析蛋白质。发现这种纯化方法的膜部分回收率为22%，膜部分中蔗糖酶的比活性增加了19倍。聚丙烯酰胺凝胶电泳后的微绒毛膜蛋白分离成21条带，其中大多数分子量高于70000。发现7条分子量从150000到超过340000的带是糖蛋白。