Sato T, Fukushima M, Tanaka S
Nihon Sanka Fujinka Gakkai Zasshi. 1982 Jul;34(7):854-62.
The present study was designed to investigate the endocrinological and morphological events which occurred in human granulosa cells. The results were as follows: Human granulosa cells harvested from preovulatory large follicles secreted 0.55 approximately 1.01 pg/cell/day of progesterone(P). During 7 days of culturing, the total P productive level was 3.96 +/- 0.22 pg/cell (mean +/- SE). It level per cell per 24 hours was the highest on the third day, after which the level tended to decrease. The granulosa cells obtained from the preovulatory large follicles secreted 2.12 +/- 0.41 pg/cell (mean +/- SE) of estradiol (E2) during 6 days culturing E2 productive volume per cell measured every 48 hours however tended to decrease with increasing time in the culture. The fine structure of the cultured granulosa cells had almost the same appearance on the third day as on the seventh day of the culture. Namely, they displayed numerous lipid droplets, widely dispersed Golgi complexes and mitochondria with plain cristae. However, smooth endoplasmic reticulum were only seen sporadically. Judging from the cultured results of granulosa cells, they showed an early stage of formation of corpus luteum in the fine structure which was assumed to be reflected by the endocrinological events which secreted P and E2.
本研究旨在调查人类颗粒细胞中发生的内分泌和形态学变化。结果如下：从排卵前大卵泡中采集的人类颗粒细胞每天分泌约0.55至1.01 pg/细胞的孕酮（P）。在培养7天期间，总P产生水平为3.96 +/- 0.22 pg/细胞（平均值 +/- 标准误）。每24小时每细胞的水平在第三天最高，之后该水平趋于下降。在培养6天期间，从排卵前大卵泡中获得的颗粒细胞分泌2.12 +/- 0.41 pg/细胞（平均值 +/- 标准误）的雌二醇（E2）。然而，每48小时测量的每细胞E2产生量倾向于随着培养时间的增加而下降。培养的颗粒细胞的精细结构在培养第三天和第七天几乎具有相同的外观。即，它们显示出大量脂滴、广泛分散的高尔基体复合体和具有平坦嵴的线粒体。然而，光滑内质网仅偶尔可见。从颗粒细胞的培养结果来看，它们在精细结构上显示出黄体形成的早期阶段，这被认为是由分泌P和E2的内分泌事件所反映的。
