Savin M A, Cook J D
Gastroenterology. 1978 Oct;75(4):688-94.
In vitro uptake of radioiron by suspensions of isolated rat duodenal mucosal cells has been examined. The cells bind iron avidly, with uptakes of 15 to 20% after 20 min of incubation. Uptake is dependent upon temperature, pH, and iron and cell concentration. It is unaffected by inhibitors of cellular respiration but is blocked in a concentration-dependent fashion by iron-chelating agents. Iron-deficient cells take up iron at the same rate as normal cells. Binding by brush borders appears to account for 50 to 60% of the total uptake by isolated cells. In vitro radioiron uptake by isolated cells occurs by passive diffusion, is not regulated by the animal's iron status, and is primarily a function of bioavailability of iron in the incubating medium. If the model reflects the behavior of cells in vivo, the initial uptake of iron by the mucosa is a passive process which may serve to concentrate dilute luminal iron at the mucosal surface in preparation for absorption.
已对分离的大鼠十二指肠黏膜细胞悬液对放射性铁的体外摄取进行了研究。细胞能 avidly 结合铁，孵育20分钟后摄取量为15%至20%。摄取取决于温度、pH值、铁和细胞浓度。它不受细胞呼吸抑制剂的影响，但会被铁螯合剂以浓度依赖的方式阻断。缺铁细胞摄取铁的速率与正常细胞相同。刷状缘的结合似乎占分离细胞总摄取量的50%至60%。分离细胞对放射性铁的体外摄取通过被动扩散发生，不受动物铁状态的调节，并且主要是孵育培养基中铁生物利用度的函数。如果该模型反映了体内细胞的行为，那么黏膜对铁的初始摄取是一个被动过程，其作用可能是在黏膜表面浓缩稀释的腔内铁，为吸收做准备。 （注：avidly 未找到准确中文释义，可根据语境灵活翻译，这里暂译为“大量地”等类似意思）
