Phillips D M, Shalgi R
J Exp Zool. 1982 Jul 1;221(3):373-8. doi: 10.1002/jez.1402210313.
The mechanics of sperm entry into the oocyte were examined in rat ova fertilized in vivo. With the scanning electron microscope we observe that the spermatozoon is first associated with the oocyte by the region of the convex surface of the sperm head which is immediately anterior to the postacrosomal region. At this early stage the anterior tip of the sperm head is directed away from the oocyte surface. The spermatozoon subsequently appears to rotate so that it comes to lie on its "side." The membrane over the sperm head becomes free of microvilli and a large incorporation cone is subsequently formed. It is concluded that in the rat initial fusion of sperm and egg probably occurs between the oolemma and the sperm plasmalemma over the equatorial segment of the acrosome at the convex surface of the sperm head which is just anterior to the postacrosomal region.
在体内受精的大鼠卵子中研究了精子进入卵母细胞的机制。通过扫描电子显微镜，我们观察到精子首先通过精子头部凸面区域与卵母细胞结合，该区域紧邻顶体后区域的前方。在这个早期阶段，精子头部的前端远离卵母细胞表面。随后精子似乎发生旋转，使其侧躺。精子头部上方的膜变得没有微绒毛，随后形成一个大的纳入锥。得出的结论是，在大鼠中，精子与卵子的初始融合可能发生在卵质膜和精子质膜之间，位于精子头部凸面顶体赤道段的位置，该位置恰好在顶体后区域的前方。
