Macklin W B, Lees M B
J Neurochem. 1982 Feb;38(2):348-55. doi: 10.1111/j.1471-4159.1982.tb08635.x.
Two solid-phase immunoassays have been developed for quantitation of antibodies to bovine white matter proteolipid apoprotein. Conditions were established for optimal specific antibody binding. Water-soluble proteolipid apoprotein was bound to microtiter plates and plates were incubated with test serum. Goat anti-rabbit IgG conjugated with horseradish peroxidase was used as the second antibody for an enzyme-linked immunospecific assay and 125I-labeled protein A for a radioimmunoassay. Both procedures have been used to follow the time course of anti-proteolipid antibody production in rabbits and to compare different immunization protocols.
已开发出两种固相免疫测定法来定量检测抗牛白质蛋白脂质载脂蛋白的抗体。确定了最佳特异性抗体结合的条件。将水溶性蛋白脂质载脂蛋白结合到微量滴定板上，然后将板与测试血清一起孵育。与辣根过氧化物酶结合的山羊抗兔IgG用作酶联免疫特异性测定的第二抗体，125I标记的蛋白A用作放射免疫测定的第二抗体。这两种方法都已用于追踪兔体内抗蛋白脂质抗体产生的时间进程，并比较不同的免疫方案。
