Lees M B
Biomedical Sciences Division, E.K. Shriver Center, Waltham, MA 02254, USA.
Neurochem Res. 1998 Mar;23(3):261-71. doi: 10.1023/a:1022488912996.
This review is a personal memoir of the history of proteolipids and is limited to aspects of the field with which the author has been involved in one way or another. The discovery of proteolipids was a serendipitous observation made in the course of the study of sulfatides. Initial focus was on the chemical characterization of brain proteolipids, their behavior under different conditions and their identification as the major protein of CNS myelin. The sequence of PLP was obtained using solid phase protein sequencing techniques. This, in turn, made possible a new era in which biochemical, cellular and molecular approaches could be applied to address new questions about PLP. Identification of genetic defects in the PLP molecule and its regulation has contributed to understanding myelin biology. Studies of the encephalitogenic activity of PLP in animal models have influenced the views of inflammatory processes in multiple sclerosis. Despite remarkable progress, much remains to be learned about the structure and function of PLP.
本综述是关于蛋白脂质历史的个人回忆录，仅限于作者以某种方式参与过的该领域的各个方面。蛋白脂质的发现是在研究硫脂过程中偶然观察到的。最初的重点是脑蛋白脂质的化学特征、它们在不同条件下的行为以及它们作为中枢神经系统髓鞘主要蛋白质的鉴定。使用固相蛋白质测序技术获得了PLP的序列。这反过来又开启了一个新时代，在这个时代中，可以应用生化、细胞和分子方法来解决关于PLP的新问题。PLP分子中遗传缺陷的鉴定及其调控有助于理解髓鞘生物学。在动物模型中对PLP致脑炎活性的研究影响了对多发性硬化症炎症过程的看法。尽管取得了显著进展，但关于PLP的结构和功能仍有许多有待了解之处。
