Tyson C A, Dilley J V, Sasmore D P, Spanggord R J, Newell G W, Dacre J C
J Toxicol Environ Health. 1982 Apr;9(4):545-64. doi: 10.1080/15287398209530187.
The single-dose and repeated-exposure toxicity of a synthetic mixture of 30 nitrotoluene analogs, representative of a complex industrial wastewater termed condensate water, was evaluated in dogs, rats, and mice. The single-dose oral LD50s for the synthetic condensate water (CW) were 447 and 295 mg/kg in male and female rats, respectively. In the repeated-exposure studies, dogs were given 0, 0.05, 0.5, or 5 mg of CW per kilogram of body weight by capsule daily for 26 wk. Rats and mice received 0, 0.001, 0.01, or 0.1% of this mixture in their diet for 4 or 13 wk. Groups of each rodent species were set aside for 4 wk to assess recovery. The most notable findings were a compensatory anemia with reticulocytosis (severe in rats), Heinz body formation, and related blood cell abnormalities and hemosiderin in the spleen; pigmentation in the liver cells; atrophy and aspermia in the testes; hyperplasia and inflammation in the female reproductive organs; and neurotoxic signs at the high doses. Rats and mice also experienced food intake and body weight depression and exhibited some alterations in organ weights (spleen, testes, and liver). The findings were referable principally to the two major components 2,4,- and 2,6-dinitrotoluene, but the "no observable effects" levels were lower for the mixture.
对一种由30种硝基甲苯类似物组成的合成混合物进行了单剂量和重复暴露毒性评估，该混合物代表一种称为冷凝水的复杂工业废水。实验对象为犬、大鼠和小鼠。合成冷凝水（CW）对雄性和雌性大鼠的经口单剂量半数致死量（LD50）分别为447和295毫克/千克。在重复暴露研究中，犬每天通过胶囊给予每千克体重0、0.05、0.5或5毫克的CW，持续26周。大鼠和小鼠在其饮食中摄入0、0.001、0.01或0.1%的该混合物，持续4周或13周。每种啮齿动物物种的部分组被留出4周以评估恢复情况。最显著的发现包括伴有网织红细胞增多的代偿性贫血（大鼠中严重）、海因茨小体形成、相关血细胞异常以及脾脏中的含铁血黄素；肝细胞色素沉着；睾丸萎缩和无精子症；雌性生殖器官增生和炎症；以及高剂量时的神经毒性体征。大鼠和小鼠还出现食物摄入量和体重下降，并表现出一些器官重量（脾脏、睾丸和肝脏）的改变。这些发现主要归因于两种主要成分2,4 - 和2,6 - 二硝基甲苯，但混合物的“无明显影响”水平更低。
