Damon M, Chavis C
J Steroid Biochem. 1982 Jun;16(6):771-8. doi: 10.1016/0022-4731(82)90034-6.
Four new metabolites and their fatty acid derivatives have been identified as components of the steroid metabolic pathway in rat embryo fibroblasts grown during the confluent phase in the presence of [4-14C]-pregnenolone and [4-14C]-progesterone. These compounds are 3 alpha, 20 alpha and 3 beta, 20 alpha-dihydroxy-5 alpha-pregnanes, 4-pregnene-3 alpha, 20 alpha-diol and 5 alpha-pregnane-3 beta, 20 alpha, 21-triol. The labelled free and esterified steroids synthetized in the cells were released into the culture medium. The purpose of this study was to demonstrate that, besides t.l.c. enzymatic and chemical reactions, g.l.c./mass spectrometry coupling is a powerful tool for identifying the structures of sub-microgram amounts of radiolabelled steroids. The consequent metabolic features are discussed in terms of steroid cellular biosynthesis and kinetics.
在汇合期，于存在[4-¹⁴C]-孕烯醇酮和[4-¹⁴C]-孕酮的条件下培养的大鼠胚胎成纤维细胞中，已鉴定出四种新的代谢物及其脂肪酸衍生物是类固醇代谢途径的组成部分。这些化合物为3α,20α-二羟基-5α-孕烷、3β,20α-二羟基-5α-孕烷、4-孕烯-3α,20α-二醇和5α-孕烷-3β,20α,21-三醇。细胞中合成的标记游离类固醇和酯化类固醇被释放到培养基中。本研究的目的是证明，除了薄层层析、酶促和化学反应外，气相色谱/质谱联用是鉴定亚微克量放射性标记类固醇结构的有力工具。根据类固醇细胞生物合成和动力学对相应的代谢特征进行了讨论。
