Docchio F, Ramponi R, Sacchi C A, Bottiroli G, Freitas I
Lasers Surg Med. 1982;2(1):21-8. doi: 10.1002/lsm.1900020103.
This work presents measurements of time-resolved fluorescence microscopy of hematoporphyrin-derivative (HpD) in single cells of mice tissue (both tumor and normal cells), in HeLa Cells, and in solution. The measurements were performed using a pulsed-laser microfluorometer with high spatial and temporal resolution. In agreement with the results obtained with other techniques, it has been found that the tumor cells examined present an HpD uptake about five times higher than that of the normal cells of the corresponding tissue and that, within a cell, HpD become localized mainly in the cytoplasm. It has also been found that the fluorescence decay time is different in cells as compared with solution, and that the presence of HpD stabilizes cell auto-fluorescence. These results are discussed.
这项工作展示了对小鼠组织（肿瘤细胞和正常细胞）、HeLa细胞以及溶液中单个细胞内血卟啉衍生物（HpD）的时间分辨荧光显微镜测量结果。测量使用了具有高空间和时间分辨率的脉冲激光显微荧光计。与其他技术获得的结果一致，已发现所检测的肿瘤细胞摄取的HpD比相应组织的正常细胞高约五倍，并且在细胞内，HpD主要定位于细胞质中。还发现细胞中的荧光衰减时间与溶液中的不同，并且HpD的存在使细胞自发荧光稳定。对这些结果进行了讨论。
