Majewski H, Hedler L, Steppeler A, Starke K
Naunyn Schmiedebergs Arch Pharmacol. 1982 May;319(2):125-9. doi: 10.1007/BF00503924.
The outflow of noradrenaline, 3,4-dihydroxyphenylglycol (DOPEG) and 3,4-dihydroxymandelic acid (DOMA) from rabbit perfused hearts was studied by chromatography on alumina followed by high pressure liquid chromatography with electrochemical detection. In the absence of drugs and without nerve stimulation, the outflow of endogenous noradrenaline over a period of 108 min averaged 0.17 pmol X g-1 X min-1 and the outflow of DOPEG 2.1 pmol X g-1 X min-1. The outflow of DOMA was below the detection limit (less than 0.13 pmol X g-1 X min-1). The effect of perfusion with (-)-noradrenaline 0.1, or 10 mumol/l for 18 min was then investigated. As the concentration of nor-adrenaline increased so did the outflow of DOPEG. Moreover, DOMA was found in the venous effluent during and after perfusion with noradrenaline 1 or 10 mumol/l. The increase in the outflow of DOPEG and DOMA was almost abolished when cocaine 10 mumol/l was present during the perfusion with noradrenaline 1 mumol/l. The release of endogenous noradrenaline by sympathetic nerve stimulation or tyramine 10 mumol/l, but not the release evoked by nicotine 30 mumol/l, was accompanied by an increase in the outflow of DOPEG; an outflow of DOMA was not observed. It is concluded that, in the rabbit perfused heart, DOPEG is an important metabolite of endogenous noradrenaline. DOMA is at best a minor product, either when the neurones are at rest or when noradrenaline is released by sympathetic nerve stimulation, nicotine or tyramine. DOMA is formed in detectable amounts when the tissue is exposed to a high concentration of exogenous noradrenaline. Like DOPEG, it is formed intraneuronally. The results confirm and extend those obtained previously on guinea-pig incubated atria. They make it unlikely that, in these tissues at least, DOMA formation is one of the physiological pathways of noradrenaline catabolism.
采用氧化铝柱色谱法，随后进行带电化学检测的高压液相色谱法，研究了兔离体灌注心脏中去甲肾上腺素、3,4 - 二羟基苯乙二醇（DOPEG）和3,4 - 二羟基扁桃酸（DOMA）的流出情况。在无药物且无神经刺激的情况下，108分钟内内源性去甲肾上腺素的平均流出量为0.17 pmol·g⁻¹·min⁻¹，DOPEG的流出量为2.1 pmol·g⁻¹·min⁻¹。DOMA的流出量低于检测限（小于0.13 pmol·g⁻¹·min⁻¹）。接着研究了用0.1、1或10 μmol/L的（ - ） - 去甲肾上腺素灌注18分钟的效果。随着去甲肾上腺素浓度的增加，DOPEG的流出量也增加。此外，在用1或10 μmol/L去甲肾上腺素灌注期间及之后，在静脉流出液中发现了DOMA。当在灌注1 μmol/L去甲肾上腺素期间加入10 μmol/L可卡因时，DOPEG和DOMA流出量的增加几乎被消除。交感神经刺激或10 μmol/L酪胺引起的内源性去甲肾上腺素释放，而非30 μmol/L尼古丁引起的释放，伴随着DOPEG流出量的增加；未观察到DOMA的流出。结论是，在兔离体灌注心脏中，DOPEG是内源性去甲肾上腺素的重要代谢产物。无论是神经元处于静息状态时还是交感神经刺激、尼古丁或酪胺释放去甲肾上腺素时，DOMA充其量只是次要产物。当组织暴露于高浓度外源性去甲肾上腺素时，可检测到DOMA的形成。与DOPEG一样，它在神经元内形成。这些结果证实并扩展了先前在豚鼠离体心房上获得的结果。它们表明，至少在这些组织中，DOMA的形成不太可能是去甲肾上腺素分解代谢的生理途径之一。
