仓鼠催乳素：通过同源放射免疫测定法测得的血液和垂体浓度的生理变化。

Hamster prolactin: physiological changes in blood and pituitary concentrations as measured by a homologous radioimmunoassay.

作者信息

Borer K T, Kelch R P, Corley K

出版信息

Neuroendocrinology. 1982;35(1):13-21. doi: 10.1159/000123348.

Abstract

A specific and sensitive homologous radioimmunoassay (RIA) for determination of hamster prolactin is described and compared to heterologous hamster prolactin RIA and to the homologous and heterologous RIAs for rat prolactin. With this method, we have determined that, in the golden hamster, diurnal plasma prolactin fluctuations have a mean interpeak interval of 1.5 h, that serum prolactin concentrations are influenced by day length, by ether vapors, by animal's gender, and by refeeding following a fast but not by neurosurgical procedures which increase somatic growth and serum GH concentration in adult hamsters. Variations in day length influenced pituitary prolactin content and concentration, and pituitary removal abolished prolactin secretory responses to ether stress. A heterologous RIA for hamster prolactin which utilizes radioiodinated rat prolactin and rabbit antihamster prolactin serum (RK1-15) represents the most advantageous method for the measurement of hamster prolactin because of its high sensitivity (0.14 ng/ml), high antiserum binding to iodinated rat prolactin, and the ability to measure both hamster and rat prolactin.

摘要

本文描述了一种用于测定仓鼠催乳素的特异性和灵敏的同源放射免疫分析（RIA）方法，并将其与异源仓鼠催乳素RIA以及大鼠催乳素的同源和异源RIA进行了比较。通过这种方法，我们确定，在金黄仓鼠中，血浆催乳素的昼夜波动平均峰间间隔为1.5小时，血清催乳素浓度受日长、乙醚蒸汽、动物性别以及禁食后再喂食的影响，但不受增加成年仓鼠体细胞生长和血清生长激素浓度的神经外科手术的影响。日长变化影响垂体催乳素含量和浓度，垂体切除消除了催乳素对乙醚应激的分泌反应。一种利用放射性碘化大鼠催乳素和兔抗仓鼠催乳素血清（RK1-15）的异源仓鼠催乳素RIA，因其高灵敏度（0.14 ng/ml）、抗血清与碘化大鼠催乳素的高结合力以及能够同时测量仓鼠和大鼠催乳素，是测量仓鼠催乳素最有利的方法。