犬血浆中生长激素的快速放射免疫测定法。

A rapid radioimmunoassay method of growth hormone in dog plasma.

作者信息

Cocola F, Udeschini G, Secchi C, Panerai A E, Neri P, Müller E E

出版信息

Proc Soc Exp Biol Med. 1976 Jan;151(1):140-5. doi: 10.3181/00379727-151-39161.

Abstract

A rapid and sensitive homologous radioimmunoassay (RIA) system is described for the measurement of growth hormone (GH) in dog plasma. The method requires only 40 hr and is able to detect concentrations of GH as low as 1.0 ng/ml of dog plasma. Antiserum to canine growth hormone (cGH) prepared in monkeys, exhibited a complete cross-reactivity with porcine GH, suggesting that the latter can substitute cGH in a heterologous radioimmunoassay for cGH. Studies on GH regulation performed with this RIA in the unanesthetized dog showed that this species resembles the primate more than the rat or the rabbit.

摘要

本文描述了一种快速灵敏的同源放射免疫分析（RIA）系统，用于测量犬血浆中的生长激素（GH）。该方法仅需40小时，能够检测低至1.0 ng/ml犬血浆的GH浓度。用猴制备的抗犬生长激素（cGH）抗血清与猪GH具有完全交叉反应性，这表明在异源放射免疫分析中猪GH可替代cGH。用该RIA对未麻醉犬进行的GH调节研究表明，该物种与灵长类动物的相似性超过大鼠或兔子。