神经元图谱绘制：一种光氧化反应使荧光黄适用于电子显微镜检查。
Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy.
作者信息
Maranto A R
出版信息
Science. 1982 Sep 3;217(4563):953-5. doi: 10.1126/science.7112109.
PMID:7112109
Abstract
Irradiation Lucifer yellow-filled neurons with intense blue light in the presence of 3,3'-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when cobalt or horseradish peroxidase injections are difficult or when a second intracellular marker is needed to demonstrate neuronal contacts.
摘要
在3,3'-二氨基联苯胺存在的情况下，用强蓝光照射充满路西法黄的神经元，会在注入的细胞内产生一种电子不透明的嗜锇聚合物。当钴或辣根过氧化物酶注射困难或需要第二种细胞内标记物来证明神经元联系时，这项技术很有价值。
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