Venker P, Wittmann W, Solisch P, Drescher B, Rössler H
Arch Exp Veterinarmed. 1977;31(2):317-22.
This exogenic test for the detection of enzyme activities (revertase) is helpful in differentiating between leucocytes of leukaemic cattle, on the one hand, and those of intact animals, on the other. The mechanism of such identification is based on differences in enzyme activity in the presence of poly rA/oligo dT and poly dA/oligo dT. The best results were obtained from short-time cultured leucocytes which gave ten times higher margins of incorporation by comparison between intact and preleucotic cattle. The morphological findings obtained by electron microscopy confirmed the results by other authors and enlarged them by determination, for the first time, of RNA filaments and protein rings.
这种用于检测酶活性(转化酶)的外源性试验有助于区分患白血病牛的白细胞和健康动物的白细胞。这种识别机制基于在聚rA/寡聚dT和聚dA/寡聚dT存在下酶活性的差异。通过对短期培养的白细胞进行检测,获得了最佳结果,与健康牛和白血病前期牛相比,其掺入率高出十倍。通过电子显微镜获得的形态学结果证实了其他作者的结果,并首次通过测定RNA细丝和蛋白质环对其进行了扩展。