用植物血凝素刺激马、猪、绵羊和人的外周血淋巴细胞。
Stimulation by phytohaemagglutinin of peripheral blood lymphocytes from horse, pig, sheep and man.
作者信息
Peters G J, Veerkamp J H
出版信息
Vet Immunol Immunopathol. 1982 May;3(3):295-300. doi: 10.1016/0165-2427(82)90003-4.
Optimal conditions for stimulation by phytohaemagglutinin (PHA) were established for equine, porcine, ovine and human lymphocytes in MEMS medium. Optimal thymidine concentration was determined for assay of cell transformation. With all species tested horse serum gave highest thymidine incorporation. Homologous serum was not more appropriate for lymphocytes of man, pig and sheep. Optimal stimulation was achieved at 20, 0.5-5, 5, and 10-40 micrograms PHA per 10(6) cells for human, equine, porcine and ovine lymphocytes, respectively.
在MEMS培养基中确定了植物血凝素（PHA）刺激马、猪、羊和人淋巴细胞的最佳条件。测定了用于细胞转化分析的最佳胸腺嘧啶核苷浓度。在所有测试的物种中，马血清的胸腺嘧啶核苷掺入量最高。同种血清对人、猪和羊的淋巴细胞并不更合适。对于人、马、猪和羊淋巴细胞，每10⁶个细胞分别加入20、0.5 - 5、5和10 - 40微克PHA时可实现最佳刺激。
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