纯化的绵羊外周血淋巴细胞对植物血凝素-M的体外反应。
In vitro response of purified ovine peripheral blood lymphocytes to phytohemagglutinin-M.
作者信息
Stirtzinger T, Valli V E, Jacobs R M, Beiner L, Jansen B
出版信息
Can J Vet Res. 1986 Jan;50(1):115-7.
Peripheral blood was collected from three normal yearling castrated male lambs. Lymphocytes were isolated by Ficoll-Hypaque gradient centrifugation. Lymphocyte response to phytohemagglutinin was evaluated in an isotopic uptake stimulation test under varying culture conditions in order to standardize the assay. Results were analyzed as log10 counts per minute and stimulation indices. Culture conditions consisting of 2 microL (20 micrograms) phytohemagglutinin-M/culture, 1 X 10(6) cells/mL and a 72 hour incubation period were selected for use in further studies.
从三只正常的一岁去势雄性羔羊采集外周血。通过Ficoll-泛影葡胺梯度离心法分离淋巴细胞。为使检测标准化，在不同培养条件下的同位素摄取刺激试验中评估淋巴细胞对植物血凝素的反应。结果以每分钟的log10计数和刺激指数进行分析。选择由2微升（20微克）植物血凝素-M/培养物、1×10⁶个细胞/毫升和72小时孵育期组成的培养条件用于进一步研究。
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