Metelitza D I, Eryomin A N, Usanov S A
Acta Biol Med Ger. 1982;41(1):17-21.
Kinetics of thermal inactivation of the rabbit liver microsomal cytochrome P-450 has been studied in the temperature range of 45-54 degrees C with increasing concentrations of glycerol. For all temperatures studied in the absence and presence of glycerol, thermal inactivation of cytochrome P-450 is characterized by two phases, the first being described by the first order rate constants. Glycerol markedly lowers the rate of thermal inactivation of cytochrome P-450 as well as the activation parameters delta H and delta S of the process of thermal destruction. There is a linear relationship between decreasing values of delta H, delta S and increasing glycerol concentrations in the medium.
在45-54摄氏度的温度范围内，随着甘油浓度的增加，研究了兔肝微粒体细胞色素P-450的热失活动力学。对于在不存在和存在甘油的情况下所研究的所有温度，细胞色素P-450的热失活具有两个阶段，第一阶段由一级速率常数描述。甘油显著降低了细胞色素P-450的热失活速率以及热破坏过程的活化参数ΔH和ΔS。介质中ΔH、ΔS的降低值与甘油浓度的增加之间存在线性关系。
