[Effect of temperature on the interaction of cytochrome P-450 with a variety of amines].

The interaction of cytochrome P-450 of rat liver microsomes with six amines have been investigated in Tris-HCL buffer pH 7.4 within the temperature range of 20--37 degrees C by the differential spectrophotometry method. Dissociation constants for the amine-cytochrome-P-450 complexes have been determined. The interaction of type I substrate, 1,2,7-trimethyl-decahydroquinolone-4, is characterized by the value of Ks(I)=4.14 exp (--6250/RT) mole/1. A value of Ks(II)=10(-8) exp (+6500/RT) mole/1 has been obtained for type II substrate, monomethylaniline. Association of 1,2,7-trimethyldecahydroquinolone-4 to cytochrome P-450 decreases with temperature, where as with monomethylaniline the reverse tendency is observed. Thermodynamic parameters delta H, delta F and delta S characterising the interaction of amines with cytochrome P-450 are evaluated.