Akhrem A A, Bel'skiĭ S M, Metelitsa D I
Biokhimiia. 1977 Dec;42(12):2116-20.
The interaction of cytochrome P-450 of rat liver microsomes with six amines have been investigated in Tris-HCL buffer pH 7.4 within the temperature range of 20--37 degrees C by the differential spectrophotometry method. Dissociation constants for the amine-cytochrome-P-450 complexes have been determined. The interaction of type I substrate, 1,2,7-trimethyl-decahydroquinolone-4, is characterized by the value of Ks(I)=4.14 exp (--6250/RT) mole/1. A value of Ks(II)=10(-8) exp (+6500/RT) mole/1 has been obtained for type II substrate, monomethylaniline. Association of 1,2,7-trimethyldecahydroquinolone-4 to cytochrome P-450 decreases with temperature, where as with monomethylaniline the reverse tendency is observed. Thermodynamic parameters delta H, delta F and delta S characterising the interaction of amines with cytochrome P-450 are evaluated.
在20-37摄氏度范围内，于pH 7.4的Tris-HCL缓冲液中，采用差示分光光度法研究了大鼠肝脏微粒体细胞色素P-450与六种胺类的相互作用。已测定了胺-细胞色素-P-450复合物的解离常数。I型底物1,2,7-三甲基十氢喹诺酮-4的相互作用特征为Ks(I)=4.14 exp (--6250/RT) 摩尔/升。II型底物单甲基苯胺的Ks(II)=10(-8) exp (+6500/RT) 摩尔/升。1,2,7-三甲基十氢喹诺酮-4与细胞色素P-450的结合随温度降低，而单甲基苯胺则观察到相反的趋势。评估了表征胺类与细胞色素P-450相互作用的热力学参数ΔH、ΔF和ΔS。
