Emons G, Ball P
Acta Endocrinol (Copenh). 1982 May;100(1):105-8. doi: 10.1530/acta.0.1000105.
This study was designed to test the ability of 2- and 4-hydroxyoestrogens to induce ovulations and the formation of corpora lutea in immature female rats. To this end 25 day old animals received a single injection of different doses of either 4-hydroxyoestradiol-dibenzoate (10, 25, 50 micrograms) or 2-hydroxyoestradiol-dibenzoate (25, 250 micrograms) or oestradiol-benzoate (10, 25, 60 micrograms). On day 31 the ovaries were checked for corpora lutea. 4-Hydroxyoestradiol-dibenzoate and oestradiol-benzoate in doses of 50 or 25 micrograms significantly increased the number of animals with corpora lutea whereas animals treated with 10 micrograms of either steroid did not differ significantly fron the respective vehicle groups. 2-Hydroxyoestradiol-dibenzoate, even at the high dose of 250 micrograms did not show a significant effect. This is the first demonstration that a catecholoestrogen - 4-hydroxyoestradiol - can induce ovulation. As its potency in doing so is similar to that of oestradiol and as 4-hydroxyoestradiol can be formed in neuroendocrine tissues from primary oestrogens, we conclude that this catecholoestrogen might play a role in the regulation of ovulation.
本研究旨在测试2-羟基雌激素和4-羟基雌激素诱导未成熟雌性大鼠排卵及黄体形成的能力。为此，给25日龄的动物单次注射不同剂量的4-羟基雌二醇二苯甲酸酯（10、25、50微克）或2-羟基雌二醇二苯甲酸酯（25、250微克）或苯甲酸雌二醇（10、25、60微克）。在第31天检查卵巢中的黄体。50微克或25微克剂量的4-羟基雌二醇二苯甲酸酯和苯甲酸雌二醇显著增加了有黄体的动物数量，而用10微克任一甾体处理的动物与各自的赋形剂组相比无显著差异。即使是250微克的高剂量2-羟基雌二醇二苯甲酸酯也未显示出显著效果。这是首次证明儿茶酚雌激素——4-羟基雌二醇——能诱导排卵。由于其诱导排卵的效力与雌二醇相似，且4-羟基雌二醇可由初级雌激素在神经内分泌组织中形成，我们得出结论，这种儿茶酚雌激素可能在排卵调节中起作用。
