Klein H G
Am J Pediatr Hematol Oncol. 1982 Summer;4(2):162-5.
Transfusion-induced hemochromatosis is a potential therapeutic complication of the management of sickle cell anemia. Selective transfusion with young erythrocytes (neocytes), which theoretically have a longer life-span than do regular donor red cells, could decrease transfusion frequency and reduce iron deposition in the sickle cell patient. Using blood cell separators, a method has been devised to prepare neocyte units for transfusion. Neocyte enrichment was evaluated by reticulocyte count and mean red cell age was estimated from erythrocyte pyruvate kinase, an age-dependent enzyme. These neocyte units have an average estimated men cell age of 30 days compared to 60 days for unfractionated units. Radiochromium studies in seven splenectomized thalassemia major patients revealed an average half-life of 47.4 days for neocytes stored in the frozen state compared to an average half-life of 29.5 days for standard frozen red cells. Neocyte aliquots collected from three normal, eusplenic donors had an average estimated mean cell age of 6.5 days and an average half-life of 57.2 days when reinfused. Neocyte units cost +300 to collect and process compared to $92 for frozen red cells, but use of neocyte units could reduce by one-half the transfusion requirement in transfusion-dependent patients.
输血诱导的血色素沉着症是镰状细胞贫血治疗中一种潜在的治疗并发症。选择性输注年轻红细胞（新生细胞），理论上其寿命比常规供体红细胞长，这可能会降低输血频率并减少镰状细胞患者体内的铁沉积。利用血细胞分离机，已设计出一种制备用于输血的新生细胞单位的方法。通过网织红细胞计数评估新生细胞富集情况，并根据红细胞丙酮酸激酶（一种随年龄变化的酶）估算平均红细胞年龄。这些新生细胞单位的平均估算平均细胞年龄为30天，而未分离单位为60天。对7例脾切除的重型地中海贫血患者进行的放射性铬研究显示，冷冻保存的新生细胞平均半衰期为47.4天，而标准冷冻红细胞的平均半衰期为29.5天。从3名正常、脾脏正常的供体采集的新生细胞等分样本，回输时平均估算平均细胞年龄为6.5天，平均半衰期为57.2天。采集和处理新生细胞单位的成本为300美元以上，而冷冻红细胞为92美元，但使用新生细胞单位可使依赖输血患者的输血需求减少一半。
