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在气球搭载实验中四膜虫的增殖动力学

Proliferation kinetics of paramecium tetraurelia in balloon-borne experiments.

作者信息

Croute F, Soleilhavoup J P, Vidal S, Rousseille R, Planel H

出版信息

Aviat Space Environ Med. 1982 Jun;53(6):531-6.

PMID:7115238
Abstract

Experiments were carried out to demonstrate the effect of cosmic radiation, at a balloon-flight ceiling of about 36,500 m (120,000 ft) on single-cell organism proliferation. Paramecium tetraurelia were placed in air-tight containers and maintained at 25 degrees +/- 0.1 degrees C. Cellular growth was determined by cell count, either after recovery or during the flight, by means of an automatic fixation device. Dosimetry was performed by a tissue equivalent proportional counter and was of about 0.5 mrad/h. Flight ceiling duration ranged from 48 min - 22 h. A secondary stimulating effect of growth rate, preceded by a temporary decrease, was observed after recovery. Because of the high bacterial concentration in the trans-Mediterranean flight culture medium, the temporary drop of the growth rate, due to the radiolysis products, disappears. We consider that the stimulating effect can be the result of enzymatic intracellular scavenging of radiolysis products generated in the cell.

摘要

进行了实验以证明在约36500米(120000英尺)的气球飞行高度下宇宙辐射对单细胞生物增殖的影响。将四膜虫置于气密容器中,并保持在25摄氏度±0.1摄氏度。通过自动固定装置,在恢复后或飞行期间通过细胞计数来确定细胞生长。剂量测定由组织等效比例计数器进行,约为0.5毫拉德/小时。飞行高度持续时间为48分钟至22小时。恢复后观察到生长速率有二次刺激效应,之前有暂时下降。由于跨地中海飞行培养基中细菌浓度高,由辐射分解产物导致的生长速率暂时下降消失。我们认为这种刺激效应可能是细胞内酶对细胞中产生的辐射分解产物进行清除的结果。

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Aviat Space Environ Med. 1982 Jun;53(6):531-6.
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