Adzamli I K, Kim H O, Sykes A G
Biochem J. 1982 Jun 1;203(3):669-73. doi: 10.1042/bj2030669.
The 1 : 1 reactions of three neutral Co(III) oxidants, Co(acac)3, Co(NH3)3(NO2)3 and Co(acac)2(NH3)(NO2), with reduced parsley (Petroselinum crispum) [2Fe--2S] ferredoxin (which carries a substantial negative charge), have been studied at 25 degrees C, pH 8.0 (Tris/HCl), I0.10 (NaCl). Whereas it has previously been demonstrated that with Co(NH3)6+ as oxidant the reaction if completely blocked by redox-inactive Cr(NH3)63+, the neutral oxidants are only partially blocked by this same complex. The effects of three Cr(III) complexes, Cr(NH3)63+%, Cr(en)33+ and (en)2Cr . mu(OH,O2CCH3) . CR(en)24+ have been investigated. Kinetic data for the response of 3+, neutral, as well as 1--oxidants to the presence of 3+ (and 4+) Cr(III) complexes can now be rationalized in terms of a single functional site on the protein for electron transfer. Electrostatics have a significant influence on association at this site.
在25℃、pH 8.0（Tris/HCl）、I 0.10（NaCl）条件下，研究了三种中性Co(III)氧化剂Co(acac)3、Co(NH3)3(NO2)3和Co(acac)2(NH3)(NO2)与还原型欧芹[2Fe-2S]铁氧化还原蛋白（带有大量负电荷）的1:1反应。此前已证明，以Co(NH3)6+为氧化剂时，该反应会被氧化还原惰性的Cr(NH3)63+完全阻断，而中性氧化剂仅被同一配合物部分阻断。研究了三种Cr(III)配合物Cr(NH3)63+、Cr(en)33+和(en)2Cr·μ(OH,O2CCH3)·CR(en)24+的影响。现在，关于3+、中性以及1-氧化剂对3+（和4+）Cr(III)配合物存在的响应的动力学数据，可以根据蛋白质上用于电子转移的单个功能位点进行合理化解释。静电作用对该位点的缔合有显著影响。
