血小板在体外刺激主动脉平滑肌细胞迁移。12-L-羟基-5,8,10,14-二十碳四烯酸的参与。
Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid.
作者信息
Nakao J, Ooyama T, Chang W C, Murota S, Orimo H
出版信息
Atherosclerosis. 1982 Jun;43(2-3):143-50. doi: 10.1016/0021-9150(82)90018-1.
The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,10,14-eicosatetraenoic acid inhibited it. Purified 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 x 10(-15)-6 x 10(-13) g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.
在改良的博伊登小室中测定大鼠主动脉平滑肌细胞的迁移。平滑肌细胞在体外具有运动性，其迁移受到血小板衍生因子的刺激（呈时间和剂量依赖性）。用吲哚美辛处理血小板会导致平滑肌细胞迁移显著增加，而用5,8,10,14-二十碳四烯酸处理则会抑制其迁移。极低浓度（6×10⁻¹⁵ - 6×10⁻¹³ g/ml）的纯化12-L-羟基-5,8,10,14-二十碳四烯酸能显著刺激平滑肌细胞迁移。12-L-羟基-5,8,10,14-二十碳四烯酸诱导的运动是化学动力学的。这些发现表明花生四烯酸的血小板12-脂氧合酶产物在动脉粥样硬化早期阶段具有生理重要性。
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