Tokunaga O, Watanabe T
In Vitro Cell Dev Biol. 1987 Aug;23(8):528-34. doi: 10.1007/BF02620969.
Cultures of umbilical vein endothelial cells and smooth muscle cells were studied in a constant pressure chamber. The following results were obtained: (a) Endothelial cell growth was maximal at 80 mmHg and minimal at 0 mmHg (atmospheric pressure) for the first 2 d of incubation. However, these growth rates were reversed during the following 6 d because of steady increase in growth at 0 mmHg, and a decrease in growth at higher pressures. A degeneration of endothelial cells began at 120 mmHg and marked degeneration was noted at 160 mmHg. Growth of smooth muscle cells was not influenced by ambient pressure and a steady increase in labeled nuclei continued throughout the period of culture. (b) Elastin, stainable with tannic acid, was noted electronmicroscopically in both endothelial and smooth muscle cells. (c) Production of prostacyclin by endothelial cells was maximal at 0 mmHg and minimal at 80 mmHg, in contrast to the growth pattern of these cells. Production of thromboxane B2 by endothelial cells and prostacyclin and thromboxane B2 by smooth muscle cells was very slight and not significantly different. Although it is not known at present what mechanism acts on the vascular cells when cultured in ambient pressure, these results may indicate a new concept of the behavioral relationship between endothelial cell, smooth muscle cell, and blood pressure in vivo.
在恒压腔中研究了脐静脉内皮细胞和平滑肌细胞的培养情况。得到以下结果：(a) 在培养的最初2天，内皮细胞生长在80 mmHg时最大，在0 mmHg（大气压）时最小。然而，在接下来的6天里，这些生长速率发生了逆转，因为在0 mmHg时生长稳步增加，而在较高压力下生长减少。内皮细胞在120 mmHg时开始退化，在160 mmHg时出现明显退化。平滑肌细胞的生长不受环境压力的影响，在整个培养期间标记细胞核持续稳步增加。(b) 用鞣酸可染色的弹性蛋白在内皮细胞和平滑肌细胞中均通过电子显微镜观察到。(c) 内皮细胞产生前列环素在0 mmHg时最大，在80 mmHg时最小，这与这些细胞的生长模式相反。内皮细胞产生血栓素B2以及平滑肌细胞产生前列环素和血栓素B2的量非常少，且无显著差异。尽管目前尚不清楚在环境压力下培养时作用于血管细胞的是什么机制，但这些结果可能表明体内内皮细胞、平滑肌细胞和血压之间行为关系的一个新概念。
