Hatahara T, Seyer J M
Biochim Biophys Acta. 1982 Jun 16;716(3):377-82. doi: 10.1016/0304-4165(82)90030-7.
A fibrogenic factor which stimulates collagen production without cell proliferation of rat skin fibroblast cultures was isolated from CCl4-damaged rat liver. (1) The factor was isolated from saline extracts of CCl4-induced fibrotic rat liver and fractionated by Sephadex G-50S gel filtration and DEAE-cellulose chromatography. The original extract produced a 6-fold increase in collagen synthesis and the active factor eluted from gel filtration columns in a region corresponding to 5000 daltons. (2) The active factor was destroyed by heat (57 degrees C, 30 min), phospholipase C digestion, but was insensitive to proteolytic enzymes or phospholipase A. Chemical analysis of the partially purified factor revealed relatively high quantities of phosphorus (3%) and low quantities of protein (13.3%), neutral sugar (1.9%) and uronic acid (4.9%). The possibility of this component being a complex phospholipid containing polypeptide is suggested. (3) Fibrogenic properties of the isolated factor was enhanced by apparent oxidation in air, to a more active, yet insoluble complex. Attempts to solubilize the oxidized product completely destroyed its biological activity.
从四氯化碳损伤的大鼠肝脏中分离出一种促纤维化因子，该因子可刺激大鼠皮肤成纤维细胞培养物中的胶原蛋白生成，但不会导致细胞增殖。(1) 该因子从四氯化碳诱导纤维化的大鼠肝脏的盐提取物中分离出来，并通过葡聚糖G-50S凝胶过滤和DEAE-纤维素色谱法进行分级分离。原始提取物使胶原蛋白合成增加了6倍，活性因子从凝胶过滤柱上洗脱下来的区域对应于5000道尔顿。(2) 活性因子在57摄氏度下加热30分钟、经磷脂酶C消化后被破坏，但对蛋白水解酶或磷脂酶A不敏感。对部分纯化因子的化学分析显示，其磷含量相对较高(3%)，而蛋白质(13.3%)、中性糖(1.9%)和糖醛酸(4.9%)含量较低。提示该成分可能是一种含多肽的复合磷脂。(3) 分离出的因子在空气中明显氧化后，其促纤维化特性增强，形成一种更具活性但不溶性的复合物。试图溶解氧化产物会完全破坏其生物活性。
