四氯化碳中毒大鼠肝脏胶原蛋白的定量及代谢变化
Quantitative and metabolic changes of hepatic collagens in rats after carbon tetrachloride poisoning.
作者信息
Hirayama C, Morotomi I, Hiroshige K
出版信息
Biochem J. 1970 Jun;118(2):229-32. doi: 10.1042/bj1180229.
Abstract
- The collagen hydroxyproline in rat liver was composed of 3.5% neutral-soluble collagen, 4.9% acid-soluble collagen and 91.6% insoluble collagen. In labelling studies with [(14)C]proline in vitro, the specific radioactivities of neutral-soluble, acid-soluble and insoluble collagens in rat liver were found to be 233000, 69000 and 830d.p.m./mumol of hydroxyproline respectively after 1h. 2. During subacute carbon tetrachloride poisoning the hepatic content of insoluble collagen markedly increased, whereas those of soluble collagens did not change. During recovery from subacute poisoning hepatic contents of soluble collagens were markedly decreased. 3. After 8 weeks of carbon tetrachloride poisoning the specific radioactivities of hepatic soluble collagens increased, while that of insoluble collagen decreased. During recovery from subacute poisoning, the specific radioactivities of soluble collagens decreased to the normal range and that of insoluble collagen further decreased. 4. Hepatic collagenolytic activity solubilizing insoluble collagen, which differs from mammalian collagenase, decreased under the conditions of the subacute poisoning and also during recovery from subacute poisoning.
摘要
- 大鼠肝脏中的胶原羟脯氨酸由3.5%的中性可溶性胶原、4.9%的酸性可溶性胶原和91.6%的不溶性胶原组成。在体外以[(14)C]脯氨酸进行标记研究时，1小时后大鼠肝脏中中性可溶性、酸性可溶性和不溶性胶原的比放射性分别为233000、69000和830d.p.m./μmol羟脯氨酸。2. 在亚急性四氯化碳中毒期间，不溶性胶原的肝脏含量显著增加，而可溶性胶原的含量未发生变化。在从亚急性中毒恢复过程中，肝脏中可溶性胶原的含量显著降低。3. 四氯化碳中毒8周后，肝脏可溶性胶原的比放射性增加，而不溶性胶原的比放射性降低。在从亚急性中毒恢复过程中，可溶性胶原的比放射性降至正常范围，不溶性胶原的比放射性进一步降低。4. 溶解不溶性胶原的肝脏胶原olytic活性不同于哺乳动物胶原酶，在亚急性中毒条件下以及从亚急性中毒恢复过程中均降低。
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