Pozdniakova T M, Rybachuk V N, Vovk E v
Biokhimiia. 1982 Jun;47(6):971-6.
The fragment D-fibrin monomer complex formation was studied by ammonium sulfate fractionation of the reaction mixtures initially consisting of 0.15-4.00 mg/ml of fragment D and 1.00 mg/ml of fibrin monomer, It was shown that in these mixtures the equilibrium was reached in not more than 15 sec. Fragment D binding to fibrin dependent on the fragment concentration. The Scatchard analysis of this dependence revealed a maximum of fragment D binding to one fibrin molecule of 2.95 +/- 0.28 with a Ka value of (9.04 +/- 1.08) x 10(-5) M-1 (n = 6), thus indicating a strong fragment--fibrin affinity.
通过对最初由0.15 - 4.00mg/ml的D片段和1.00mg/ml的纤维蛋白单体组成的反应混合物进行硫酸铵分级分离，研究了D - 纤维蛋白单体复合物的形成。结果表明，在这些混合物中，不超过15秒即可达到平衡。D片段与纤维蛋白的结合取决于片段浓度。对这种依赖性的Scatchard分析显示，一个纤维蛋白分子结合D片段的最大值为2.95±0.28，Ka值为(9.04±1.08)×10(-5)M-1(n = 6)，这表明D片段与纤维蛋白具有很强的亲和力。
