Smekhova K O, Ugarova T P, Pozdniakova T M, Belitser V O
Ukr Biokhim Zh. 1977 Nov-Dec;49(6):5-11.
According to the authors' observations, mixtures of fibrin monomer and fibrinogen-derived D fragment are gradually transformed into an equilibrium system when fibrin exists partly in a solid clot form and partly as a soluble material made unclottable by the influence of fragment D. The amount of such a soluble fibrin proved to be a linear function of the D fragment concentration. Three fibrin monomer preparations obtained in different ways were used. All of them showed similar properties as to the modifications with D fragment. The solubilzing activity of D fragment in the equilibrium system could sharply be raised by increasing ionic strength or pH. It is known that these medium changes produce the same stimulation effect on D fragment, being examined as an inhibitor of fibrin monomer polymerization. But the concentration dependences of the two activities of D fragment differ in principle.
根据作者的观察，当纤维蛋白部分以固体凝块形式存在，部分以受D片段影响而不可凝的可溶性物质形式存在时，纤维蛋白单体与纤维蛋白原衍生的D片段的混合物会逐渐转化为平衡系统。这种可溶性纤维蛋白的量被证明是D片段浓度的线性函数。使用了通过不同方法获得的三种纤维蛋白单体制剂。它们在与D片段的修饰方面都表现出相似的特性。通过增加离子强度或pH值，平衡系统中D片段的溶解活性可急剧提高。众所周知，这些介质变化对作为纤维蛋白单体聚合抑制剂进行检测的D片段产生相同的刺激作用。但D片段两种活性的浓度依赖性在原则上有所不同。
