Linderkamp O, Wu P Y, Meiselman H J
Pediatr Res. 1982 Nov;16(11):964-8. doi: 10.1203/00006450-198211000-00013.
The deformability of neonatal and adult red blood cells (RBC) was studied using both unseparated and density (i.e., age) fractionated RBC. Blood samples were obtained from 10 normal newborn infants (placental blood) and 10 adults. Deformability was measured by direct microscopic observation of RBC subjected to shear stresses of 2.5-500 dynes/cm2 using a counter-rotating Rheoscope. There was no significant difference in deformability between unseparated neonatal and adult RBC at any shear stress. The 3% least dense ("youngest") RBC showed significantly decreased hemoglobin concentrations (MCHC), increased volumes (MCV) and elevated deformability at any shear stress when compared to the 3% most dense ("oldest") cells, with the values for the unseparated RBC lying between these two extremes. The least dense neonatal RBC had a significantly lower MCHC and higher deformability than the least dense adult cells; however, the most dense neonatal RBC had lost more volume than the adult cells (33 versus 17%), had a higher MCHC, and were less deformable than the most dense adult cells. This indicates that the accelerated decrease in deformability of aging neonatal RBC is related to the more pronounced increase in MCHC, which is the principal determinant of the internal viscosity of the RBC.
使用未分离的和按密度（即年龄）分级的红细胞（RBC）研究了新生儿和成人红细胞的可变形性。从10名正常新生儿（脐血）和10名成人获取血样。使用反向旋转血流显微镜通过直接显微镜观察承受2.5 - 500达因/平方厘米剪应力的红细胞来测量可变形性。在任何剪应力下，未分离的新生儿和成人红细胞的可变形性均无显著差异。与3%密度最高（“最老”）的细胞相比，3%密度最低（“最年轻”）的红细胞在任何剪应力下均显示血红蛋白浓度（MCHC）显著降低、体积（MCV）增加以及可变形性升高，未分离红细胞的值介于这两个极端值之间。密度最低的新生儿红细胞的MCHC显著低于密度最低的成人细胞，但其可变形性更高；然而，密度最高的新生儿红细胞比成人细胞丢失了更多的体积（33%对17%），具有更高的MCHC，并且其可变形性低于密度最高的成人细胞。这表明衰老新生儿红细胞可变形性的加速下降与MCHC更显著的增加有关，MCHC是红细胞内粘度的主要决定因素。
